Epidermal Growth Factor (EGF)
PRODUCT OUTLINE
​Epidermal Growth Factor (EGF, Human) is a small polypeptide that plays a central role in regulating cell proliferation, survival, and differentiation. By binding to the epidermal growth factor receptor (EGFR), it activates signalling pathways critical for tissue development, repair, and regeneration in organs such as the skin, cornea, and gastrointestinal tract.
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Immobazyme's recombinant Epidermal Growth Factor (Human), or EGF, is produced in E. coli, and purified using immobilized metal ion affinity chromatography.
Fused to a trigger factor protein for improved expression and stability, our EGF is suitable for cell culture media supplementation, advanced wound-healing studies, and applications in tissue engineering and regenerative medicine.
Effect of EGF on HaCaT cell proliferation
Figure 1. Effect of EGF (20250123-EGF) on HaCaT keratinocyte cell proliferation, tested over 48 hours.
This bar graph illustrates the effect of EGF on the proliferation of HaCaT keratinocyte cells after 48 hours. The y-axis represents the absorbance value measured at 570 nm, indicating relative cell proliferation. The x-axis presents the tested samples; a negative control of HaCaT cells in the absence of EGF, and HaCaT cells treated with 2.5 ng/mL of EGF. The results demonstrates a significant improvement in HaCaT cell proliferation when in the presence of EGF.
Fibroblast Growth Factor Basic (bFGF-2)
PRODUCT OUTLINE
Basic Fibroblast Growth Factor (FGF-2) is a signalling protein from the fibroblast growth factor family, which regulates essential biological processes such as cell proliferation, differentiation, migration, and angiogenesis. Additionally, FGF-2 plays a crucial role in embryonic development as well as tissue maintenance and regeneration.
Applications in cell culture, regenerative medicine, and stem cell research, FGF-2 supports cellular growth and maintenance in vitro.
Immobazyme’s FGF-2 is produced using advanced microbial expression in E. coli, offering high purity (>90%), enhanced stability, and superior efficacy. Available as a lyophilised powder, our FGF-2 provides an accessible, scalable, and cost-effective solution for researchers and developers in cellular and molecular biology.
Effect of FGF-2 on NIH-3T3 cell proliferation (T48)
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Figure 1. Effect of FGF-2 (20241018-FGF2) on NIH-3T3 fibroblast cell proliferation, tested over 48 hours.
An MTT assay graph for FGF-2 (LOT 20241018-FGF2) shows a sigmoidal dose-response curve, with cell proliferation of fibroblasts increasing as FGF-2 concentration rises. The X-axis (FGF-2 concentration, in ng/mL) is on a logarithmic scale, while the Y-axis represents cell viability or proliferation percentage, normalised to untreated controls. The EC50, where 50% of maximal proliferation is achieved, is 90.27 ng/mL. At low concentrations, proliferation remains near baseline, increases sharply around the EC50, and plateaus at higher concentrations, indicating saturation. This demonstrates the dose-dependent effectiveness of FGF-2 in stimulating cell growth.
Insulin-like Growth Factor 1
(IGF-1)
PRODUCT OUTLINE
Insulin-like Growth Factor 1 (IGF-1) is a peptide growth factor that plays a pivotal role in cell growth, differentiation, and survival. It is a key regulator of anabolic processes, influencing tissue development, muscle regeneration, and metabolic function. IGF-1 is essential in embryogenesis, neuroprotection, and wound healing, making it a crucial component in biomedical research.
Applications in cell culture, regenerative medicine, and tissue engineering, IGF-1 enhances cellular proliferation and viability, supporting stem cell expansion, 3D tissue modelling, and biomanufacturing applications.
Immobazyme’s IGF-1 is produced using advanced microbial expression in E. coli, ensuring high purity (>95%), superior bioactivity, and enhanced stability. Available as a lyophilised powder, our IGF-1 is a scalable and cost-effective alternative to serum-derived growth factors, optimised for research and industrial applications.
Effect of IGF-1 on MCF-7 cell proliferation.
Figure 1. Effect of IGF-1 (LOT: 20250819-IGF) on MCF-7 (3000 cells/well) proliferation over 7 days.
An MTT assay shows a sigmoidal dose–response curve, with cell proliferation increasing as IGF-1 concentration rises. The X-axis (IGF-1 concentration, ng/mL) is displayed on a logarithmic scale, while the Y-axis represents absorbance at 570 nm (background-subtracted), corresponding to proliferation relative to untreated controls. The ECâ‚…â‚€, where 50% of maximal proliferation is achieved, is approximately 55 ng/mL. At low concentrations, proliferation remains near baseline, increases sharply around the ECâ‚…â‚€, and plateaus at higher concentrations, indicating saturation. This demonstrates the dose-dependent effectiveness of IGF-1 in stimulating MCF-7 cell growth.
Transforming Growth Factor Beta (TGF-β)
PRODUCT OUTLINE
Transforming Growth Factor Beta (TGF-β) is a potent, multifunctional cytokine involved in a diverse range of biological processes, including muscle repair, embryogenesis, wound healing, connective tissue development, cell growth and differentiation, proliferation, apoptosis, and immune regulation.
​Immobazyme's recombinant TGF-β is produced using advanced microbial expression in E. coli, ensuring high purity (>80%), enhanced stability, and superior efficacy. Available as a lyophilised powder, our TGF-β provides researchers and developers in cellular and molecular biology with a scalable, cost-effective, and accessible solution.
SDS-PAGE and Coomassie staining
