Recombinant Human Fibroblast Growth Factor Basic (FGF-2)

FGF-2, or Fibroblast Growth Factor 2, is a critical signalling protein that promotes cell proliferation, differentiation, and survival in various biological systems.

Immobazyme's recombinant human FGF-2 is produced using microbial expression systems and is based on the human gene sequence.

Immobazyme’s FGF-2 (Human) is ideal for a range of cell biology and biomedical applications, including:

• Mammalian cell culture

• Stem cell research and iPSC maintenance

• Tissue engineering and regenerative medicine

• Wound healing assays

It promotes cell proliferation and supports undifferentiated cell states in various serum-free media systems.

Yes. Immobazyme offers free or discounted samples of FGF-2 (Human).

Contact us to discuss your application and sample request.

Our recombinant human FGF-2 is verified by SDS-PAGE gel after lyophilisation. It is free from animal-derived components, endotoxins, and other contaminants - making it suitable for sensitive applications and consistent results.

Purity Verification: SDS-PAGE & Coomassie staining

FGF-2 (LOT: 20241018-FGF2) run on an SDS-PAGE gel after lyophilization. A prominent band was present at ~75 kDa with ~ 90% purity.

Immobazyme’s FGF-2 (Human) is rigorously tested for biological activity using a standard cell proliferation assay (e.g., NIH 3T3).

Effect of FGF-2 on NIH-3T3 cell proliferation

Effect of FGF-2 (20241018-FGF2) on NIH-3T3 fibroblast cell proliferation, tested over 48 hours.

The recommended working concentration of FGF-2 (Human) is typically 10 ng/mL to 50 ng/mL, depending on your specific cell type, culture conditions, and application.

Lower concentrations may suffice for maintenance, while higher doses may be required for robust proliferation or differentiation control.

To reconstitute the lyophilised powder:

• Remove red safety cap from vial.

• Aspirate 1 mL of sterile milliQ water into a 1 mL sterile syringe.

• Attach a sterile needle onto the syringe and insert into the vial through the centre of the rubber stopper seal.

• Gently inject the 1 mL of water into the vial, then remove the needle and syringe.

• Invert the vial 5-10 times, or until the lyophilised sample is fully reconstituted.

• Insert the needle and syringe into the reconstituted sample vial, invert the vial and gently aspirate the sample liquid into the 1 mL syringe, being sure to collect the full volume by keeping the needle end near the rubber stopper opening.

• Inject the reconstituted 1 mL sample into a sterile microfuge tube through a 0.22 μm syringe filter (provided).

• Prepare stock concentrations in sterile microfuge tubes as per your relevant standard operating procedures, keeping in mind the avoidance of repeated freeze-thaw cycles.

• Prepare working concentration stocks in sterile microfuge tubes as per your relevant standard operating procedures. The recommended working concentration for FGF-2 is 1-100 ng/mL.

Yes. Our human FGF-2 is recombinantly expressed and animal component–free, making it fully compatible with xeno-free, serum-free, and chemically defined media systems commonly used in stem cell and regenerative research.

• The lyophilised vial can be stored at –20 °C for 12 months.

• The reconstituted protein aliquots can be stored at –20°C for 6 months.

• Once resuspended use within 1 week (storage at 4°C).